apoalert caspase Search Results


apoalert colorimetric caspase 3 assay  (TaKaRa)
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apoalert caspase  (TaKaRa)
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Apoalert Caspase, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoalert1 caspase 9 6 fluorescent assay kit  (TaKaRa)
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apoalert caspase assay plates  (Becton Dickinson)
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apoalert caspase-3 colorimetric assay kit  (Becton Dickinson)
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Role of caspases in AT2R-induced apoptosis in EJ bladder cancer cells. a AT2R-induced increase <t>in</t> <t>caspase-3</t> activity in EJ cells. Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 2 d. Following this, cells were washed and lysed, and total cellular protein was assayed for caspase-3–like activity by measuring the release of pNA from the colorimetric caspase-3 substrate Ac-DEVD-pNA. Columns, mean A405 nm from three experiments; bars, SD. * P < 0.01 vs Ad-CMV-eGFP–transduced or mock-transduced cells. b Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 48 h. Western blot analysis for expression level of cleaved caspase-8 in cell lysates. c AT2R-induced increase in cleaved caspase-3 generation in EJ cells. Cells were infected with either 200, 100 or 50 ifu/cell of Ad-G-AT2R-eGFP (lanes 1, 3 and 5, respectively), 200, 100 or 50 ifu/cell of Ad-CMV-eGFP (lanes 2,4 and 6, respectively), or were mock transduced (lane 7). At 48 h after transduction, cell protein extracts were subjected to Western blot analysis. Representative blots indicating cleaved caspase-3 and β -actin under each treatment condition. d effects of caspase inhibitors on AT2R-mediated apoptosis in EJ cells. Cells were transduced with Ad-G-AT2R-eGFP (100 ifu/cell) for 6 h followed by treatment with either the caspase-3 inhibitor Ac-DEVD-CMK (20 μmol/L), the caspase-8 inhibitor Z-IETD-FMK (20 μmol/L) or control solvent (DMSO) for 24 h. Green fluorescent cells exhibiting apoptotic morphology were counted from 10 fields per well. Columns , mean of three experiments; bars, SD. * P < 0.01 vs control (DMSO-treated) cells
Apoalert Caspase 3 Colorimetric Assay Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoalert caspase 3 activation assay kit  (Becton Dickinson)
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Becton Dickinson apoalert caspase 3 activation assay kit
Role of caspases in AT2R-induced apoptosis in EJ bladder cancer cells. a AT2R-induced increase <t>in</t> <t>caspase-3</t> activity in EJ cells. Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 2 d. Following this, cells were washed and lysed, and total cellular protein was assayed for caspase-3–like activity by measuring the release of pNA from the colorimetric caspase-3 substrate Ac-DEVD-pNA. Columns, mean A405 nm from three experiments; bars, SD. * P < 0.01 vs Ad-CMV-eGFP–transduced or mock-transduced cells. b Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 48 h. Western blot analysis for expression level of cleaved caspase-8 in cell lysates. c AT2R-induced increase in cleaved caspase-3 generation in EJ cells. Cells were infected with either 200, 100 or 50 ifu/cell of Ad-G-AT2R-eGFP (lanes 1, 3 and 5, respectively), 200, 100 or 50 ifu/cell of Ad-CMV-eGFP (lanes 2,4 and 6, respectively), or were mock transduced (lane 7). At 48 h after transduction, cell protein extracts were subjected to Western blot analysis. Representative blots indicating cleaved caspase-3 and β -actin under each treatment condition. d effects of caspase inhibitors on AT2R-mediated apoptosis in EJ cells. Cells were transduced with Ad-G-AT2R-eGFP (100 ifu/cell) for 6 h followed by treatment with either the caspase-3 inhibitor Ac-DEVD-CMK (20 μmol/L), the caspase-8 inhibitor Z-IETD-FMK (20 μmol/L) or control solvent (DMSO) for 24 h. Green fluorescent cells exhibiting apoptotic morphology were counted from 10 fields per well. Columns , mean of three experiments; bars, SD. * P < 0.01 vs control (DMSO-treated) cells
Apoalert Caspase 3 Activation Assay Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoalert-caspase-3 assay  (Promega)
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Promega apoalert-caspase-3 assay
Role of caspases in AT2R-induced apoptosis in EJ bladder cancer cells. a AT2R-induced increase <t>in</t> <t>caspase-3</t> activity in EJ cells. Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 2 d. Following this, cells were washed and lysed, and total cellular protein was assayed for caspase-3–like activity by measuring the release of pNA from the colorimetric caspase-3 substrate Ac-DEVD-pNA. Columns, mean A405 nm from three experiments; bars, SD. * P < 0.01 vs Ad-CMV-eGFP–transduced or mock-transduced cells. b Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 48 h. Western blot analysis for expression level of cleaved caspase-8 in cell lysates. c AT2R-induced increase in cleaved caspase-3 generation in EJ cells. Cells were infected with either 200, 100 or 50 ifu/cell of Ad-G-AT2R-eGFP (lanes 1, 3 and 5, respectively), 200, 100 or 50 ifu/cell of Ad-CMV-eGFP (lanes 2,4 and 6, respectively), or were mock transduced (lane 7). At 48 h after transduction, cell protein extracts were subjected to Western blot analysis. Representative blots indicating cleaved caspase-3 and β -actin under each treatment condition. d effects of caspase inhibitors on AT2R-mediated apoptosis in EJ cells. Cells were transduced with Ad-G-AT2R-eGFP (100 ifu/cell) for 6 h followed by treatment with either the caspase-3 inhibitor Ac-DEVD-CMK (20 μmol/L), the caspase-8 inhibitor Z-IETD-FMK (20 μmol/L) or control solvent (DMSO) for 24 h. Green fluorescent cells exhibiting apoptotic morphology were counted from 10 fields per well. Columns , mean of three experiments; bars, SD. * P < 0.01 vs control (DMSO-treated) cells
Apoalert Caspase 3 Assay, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoalert caspase-8 fluorescent assay kit  (Becton Dickinson)
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Role of caspases in AT2R-induced apoptosis in EJ bladder cancer cells. a AT2R-induced increase <t>in</t> <t>caspase-3</t> activity in EJ cells. Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 2 d. Following this, cells were washed and lysed, and total cellular protein was assayed for caspase-3–like activity by measuring the release of pNA from the colorimetric caspase-3 substrate Ac-DEVD-pNA. Columns, mean A405 nm from three experiments; bars, SD. * P < 0.01 vs Ad-CMV-eGFP–transduced or mock-transduced cells. b Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 48 h. Western blot analysis for expression level of cleaved caspase-8 in cell lysates. c AT2R-induced increase in cleaved caspase-3 generation in EJ cells. Cells were infected with either 200, 100 or 50 ifu/cell of Ad-G-AT2R-eGFP (lanes 1, 3 and 5, respectively), 200, 100 or 50 ifu/cell of Ad-CMV-eGFP (lanes 2,4 and 6, respectively), or were mock transduced (lane 7). At 48 h after transduction, cell protein extracts were subjected to Western blot analysis. Representative blots indicating cleaved caspase-3 and β -actin under each treatment condition. d effects of caspase inhibitors on AT2R-mediated apoptosis in EJ cells. Cells were transduced with Ad-G-AT2R-eGFP (100 ifu/cell) for 6 h followed by treatment with either the caspase-3 inhibitor Ac-DEVD-CMK (20 μmol/L), the caspase-8 inhibitor Z-IETD-FMK (20 μmol/L) or control solvent (DMSO) for 24 h. Green fluorescent cells exhibiting apoptotic morphology were counted from 10 fields per well. Columns , mean of three experiments; bars, SD. * P < 0.01 vs control (DMSO-treated) cells
Apoalert Caspase 8 Fluorescent Assay Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoalert caspase pro ling kit  (Becton Dickinson)
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Role of caspases in AT2R-induced apoptosis in EJ bladder cancer cells. a AT2R-induced increase <t>in</t> <t>caspase-3</t> activity in EJ cells. Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 2 d. Following this, cells were washed and lysed, and total cellular protein was assayed for caspase-3–like activity by measuring the release of pNA from the colorimetric caspase-3 substrate Ac-DEVD-pNA. Columns, mean A405 nm from three experiments; bars, SD. * P < 0.01 vs Ad-CMV-eGFP–transduced or mock-transduced cells. b Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 48 h. Western blot analysis for expression level of cleaved caspase-8 in cell lysates. c AT2R-induced increase in cleaved caspase-3 generation in EJ cells. Cells were infected with either 200, 100 or 50 ifu/cell of Ad-G-AT2R-eGFP (lanes 1, 3 and 5, respectively), 200, 100 or 50 ifu/cell of Ad-CMV-eGFP (lanes 2,4 and 6, respectively), or were mock transduced (lane 7). At 48 h after transduction, cell protein extracts were subjected to Western blot analysis. Representative blots indicating cleaved caspase-3 and β -actin under each treatment condition. d effects of caspase inhibitors on AT2R-mediated apoptosis in EJ cells. Cells were transduced with Ad-G-AT2R-eGFP (100 ifu/cell) for 6 h followed by treatment with either the caspase-3 inhibitor Ac-DEVD-CMK (20 μmol/L), the caspase-8 inhibitor Z-IETD-FMK (20 μmol/L) or control solvent (DMSO) for 24 h. Green fluorescent cells exhibiting apoptotic morphology were counted from 10 fields per well. Columns , mean of three experiments; bars, SD. * P < 0.01 vs control (DMSO-treated) cells
Apoalert Caspase Pro Ling Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoalert caspase-9/6 fluorometric assay kit  (Becton Dickinson)
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Becton Dickinson apoalert caspase-9/6 fluorometric assay kit
Role of caspases in AT2R-induced apoptosis in EJ bladder cancer cells. a AT2R-induced increase <t>in</t> <t>caspase-3</t> activity in EJ cells. Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 2 d. Following this, cells were washed and lysed, and total cellular protein was assayed for caspase-3–like activity by measuring the release of pNA from the colorimetric caspase-3 substrate Ac-DEVD-pNA. Columns, mean A405 nm from three experiments; bars, SD. * P < 0.01 vs Ad-CMV-eGFP–transduced or mock-transduced cells. b Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 48 h. Western blot analysis for expression level of cleaved caspase-8 in cell lysates. c AT2R-induced increase in cleaved caspase-3 generation in EJ cells. Cells were infected with either 200, 100 or 50 ifu/cell of Ad-G-AT2R-eGFP (lanes 1, 3 and 5, respectively), 200, 100 or 50 ifu/cell of Ad-CMV-eGFP (lanes 2,4 and 6, respectively), or were mock transduced (lane 7). At 48 h after transduction, cell protein extracts were subjected to Western blot analysis. Representative blots indicating cleaved caspase-3 and β -actin under each treatment condition. d effects of caspase inhibitors on AT2R-mediated apoptosis in EJ cells. Cells were transduced with Ad-G-AT2R-eGFP (100 ifu/cell) for 6 h followed by treatment with either the caspase-3 inhibitor Ac-DEVD-CMK (20 μmol/L), the caspase-8 inhibitor Z-IETD-FMK (20 μmol/L) or control solvent (DMSO) for 24 h. Green fluorescent cells exhibiting apoptotic morphology were counted from 10 fields per well. Columns , mean of three experiments; bars, SD. * P < 0.01 vs control (DMSO-treated) cells
Apoalert Caspase 9/6 Fluorometric Assay Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoalert caspase 3  (Becton Dickinson)
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Role of caspases in AT2R-induced apoptosis in EJ bladder cancer cells. a AT2R-induced increase <t>in</t> <t>caspase-3</t> activity in EJ cells. Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 2 d. Following this, cells were washed and lysed, and total cellular protein was assayed for caspase-3–like activity by measuring the release of pNA from the colorimetric caspase-3 substrate Ac-DEVD-pNA. Columns, mean A405 nm from three experiments; bars, SD. * P < 0.01 vs Ad-CMV-eGFP–transduced or mock-transduced cells. b Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 48 h. Western blot analysis for expression level of cleaved caspase-8 in cell lysates. c AT2R-induced increase in cleaved caspase-3 generation in EJ cells. Cells were infected with either 200, 100 or 50 ifu/cell of Ad-G-AT2R-eGFP (lanes 1, 3 and 5, respectively), 200, 100 or 50 ifu/cell of Ad-CMV-eGFP (lanes 2,4 and 6, respectively), or were mock transduced (lane 7). At 48 h after transduction, cell protein extracts were subjected to Western blot analysis. Representative blots indicating cleaved caspase-3 and β -actin under each treatment condition. d effects of caspase inhibitors on AT2R-mediated apoptosis in EJ cells. Cells were transduced with Ad-G-AT2R-eGFP (100 ifu/cell) for 6 h followed by treatment with either the caspase-3 inhibitor Ac-DEVD-CMK (20 μmol/L), the caspase-8 inhibitor Z-IETD-FMK (20 μmol/L) or control solvent (DMSO) for 24 h. Green fluorescent cells exhibiting apoptotic morphology were counted from 10 fields per well. Columns , mean of three experiments; bars, SD. * P < 0.01 vs control (DMSO-treated) cells
Apoalert Caspase 3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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apoalert caspase-3 colorimetric assay kit  (Promega)
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Promega apoalert caspase-3 colorimetric assay kit
Role of caspases in AT2R-induced apoptosis in EJ bladder cancer cells. a AT2R-induced increase <t>in</t> <t>caspase-3</t> activity in EJ cells. Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 2 d. Following this, cells were washed and lysed, and total cellular protein was assayed for caspase-3–like activity by measuring the release of pNA from the colorimetric caspase-3 substrate Ac-DEVD-pNA. Columns, mean A405 nm from three experiments; bars, SD. * P < 0.01 vs Ad-CMV-eGFP–transduced or mock-transduced cells. b Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 48 h. Western blot analysis for expression level of cleaved caspase-8 in cell lysates. c AT2R-induced increase in cleaved caspase-3 generation in EJ cells. Cells were infected with either 200, 100 or 50 ifu/cell of Ad-G-AT2R-eGFP (lanes 1, 3 and 5, respectively), 200, 100 or 50 ifu/cell of Ad-CMV-eGFP (lanes 2,4 and 6, respectively), or were mock transduced (lane 7). At 48 h after transduction, cell protein extracts were subjected to Western blot analysis. Representative blots indicating cleaved caspase-3 and β -actin under each treatment condition. d effects of caspase inhibitors on AT2R-mediated apoptosis in EJ cells. Cells were transduced with Ad-G-AT2R-eGFP (100 ifu/cell) for 6 h followed by treatment with either the caspase-3 inhibitor Ac-DEVD-CMK (20 μmol/L), the caspase-8 inhibitor Z-IETD-FMK (20 μmol/L) or control solvent (DMSO) for 24 h. Green fluorescent cells exhibiting apoptotic morphology were counted from 10 fields per well. Columns , mean of three experiments; bars, SD. * P < 0.01 vs control (DMSO-treated) cells
Apoalert Caspase 3 Colorimetric Assay Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Role of caspases in AT2R-induced apoptosis in EJ bladder cancer cells. a AT2R-induced increase in caspase-3 activity in EJ cells. Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 2 d. Following this, cells were washed and lysed, and total cellular protein was assayed for caspase-3–like activity by measuring the release of pNA from the colorimetric caspase-3 substrate Ac-DEVD-pNA. Columns, mean A405 nm from three experiments; bars, SD. * P < 0.01 vs Ad-CMV-eGFP–transduced or mock-transduced cells. b Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 48 h. Western blot analysis for expression level of cleaved caspase-8 in cell lysates. c AT2R-induced increase in cleaved caspase-3 generation in EJ cells. Cells were infected with either 200, 100 or 50 ifu/cell of Ad-G-AT2R-eGFP (lanes 1, 3 and 5, respectively), 200, 100 or 50 ifu/cell of Ad-CMV-eGFP (lanes 2,4 and 6, respectively), or were mock transduced (lane 7). At 48 h after transduction, cell protein extracts were subjected to Western blot analysis. Representative blots indicating cleaved caspase-3 and β -actin under each treatment condition. d effects of caspase inhibitors on AT2R-mediated apoptosis in EJ cells. Cells were transduced with Ad-G-AT2R-eGFP (100 ifu/cell) for 6 h followed by treatment with either the caspase-3 inhibitor Ac-DEVD-CMK (20 μmol/L), the caspase-8 inhibitor Z-IETD-FMK (20 μmol/L) or control solvent (DMSO) for 24 h. Green fluorescent cells exhibiting apoptotic morphology were counted from 10 fields per well. Columns , mean of three experiments; bars, SD. * P < 0.01 vs control (DMSO-treated) cells

Journal: Journal of Experimental & Clinical Cancer Research : CR

Article Title: Angiotensin II type 2 receptor promotes apoptosis and inhibits angiogenesis in bladder cancer

doi: 10.1186/s13046-017-0542-0

Figure Lengend Snippet: Role of caspases in AT2R-induced apoptosis in EJ bladder cancer cells. a AT2R-induced increase in caspase-3 activity in EJ cells. Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 2 d. Following this, cells were washed and lysed, and total cellular protein was assayed for caspase-3–like activity by measuring the release of pNA from the colorimetric caspase-3 substrate Ac-DEVD-pNA. Columns, mean A405 nm from three experiments; bars, SD. * P < 0.01 vs Ad-CMV-eGFP–transduced or mock-transduced cells. b Cells underwent transduction with Ad-CMV-eGFP or Ad-G-AT2R-eGFP (100 ifu/cell) or were mock transduced for 48 h. Western blot analysis for expression level of cleaved caspase-8 in cell lysates. c AT2R-induced increase in cleaved caspase-3 generation in EJ cells. Cells were infected with either 200, 100 or 50 ifu/cell of Ad-G-AT2R-eGFP (lanes 1, 3 and 5, respectively), 200, 100 or 50 ifu/cell of Ad-CMV-eGFP (lanes 2,4 and 6, respectively), or were mock transduced (lane 7). At 48 h after transduction, cell protein extracts were subjected to Western blot analysis. Representative blots indicating cleaved caspase-3 and β -actin under each treatment condition. d effects of caspase inhibitors on AT2R-mediated apoptosis in EJ cells. Cells were transduced with Ad-G-AT2R-eGFP (100 ifu/cell) for 6 h followed by treatment with either the caspase-3 inhibitor Ac-DEVD-CMK (20 μmol/L), the caspase-8 inhibitor Z-IETD-FMK (20 μmol/L) or control solvent (DMSO) for 24 h. Green fluorescent cells exhibiting apoptotic morphology were counted from 10 fields per well. Columns , mean of three experiments; bars, SD. * P < 0.01 vs control (DMSO-treated) cells

Article Snippet: Caspase-3–like protease activity was assessed using the BD ApoAlert caspase-3 colorimetric assay kit (BD Biosciences) as described by the manufacturer.

Techniques: Activity Assay, Transduction, Western Blot, Expressing, Infection